In LD

Enzymes

PFL

PDHC

Genes localization

Chromosomic genes [30] .

Chromosomic genes [30] .

Activating factors/ optimal activity parameters

Anaerobic conditionsOptimal pH: superior to 7 [32] Carbon source: galactose [37] .

Oxygen [3] Lactose limitation [40] pH optimum: 5.5 - 6.5 [32] .

Inhibitory factors

Oxygen [3] [32] [39] pH inferior to 6 [3] Triose phosphates as glyceraldehyde-3-phosphate and dihydroxyacetone phosphate [34] [38] [39] .

NADHAnaerobic condition [35] .

Enzymes characteristicsa

Km for pyruvate: 1 mM at pH 7.2 [34] .

Enzyme requires to be functional: lipoic acid, divalent cations, thiamine pyrophosphate (TPP) and CoA [35] [36] Specific activity: 6.5 U/mgKm for pyruvate: 1 mMKm for TPP: 3.2 µM [35] .