In LD | Enzymes | |
PFL | PDHC | |
Genes localization | Chromosomic genes [30] . | Chromosomic genes [30] . |
Activating factors/ optimal activity parameters | Anaerobic conditionsOptimal pH: superior to 7 [32] Carbon source: galactose [37] . | Oxygen [3] Lactose limitation [40] pH optimum: 5.5 - 6.5 [32] . |
Inhibitory factors | Oxygen [3] [32] [39] pH inferior to 6 [3] Triose phosphates as glyceraldehyde-3-phosphate and dihydroxyacetone phosphate [34] [38] [39] . | NADHAnaerobic condition [35] . |
Enzymes characteristicsa | Km for pyruvate: 1 mM at pH 7.2 [34] . | Enzyme requires to be functional: lipoic acid, divalent cations, thiamine pyrophosphate (TPP) and CoA [35] [36] Specific activity: 6.5 U/mgKm for pyruvate: 1 mMKm for TPP: 3.2 µM [35] . |